Recombinant RTN4RL2 抗体 (AA 279-420)
Quick Overview for Recombinant RTN4RL2 抗体 (AA 279-420) (ABIN5668205)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 279-420
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原理
- Anti-NgR2 [P14], Rabbit IgG, kappa
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特异性
- The antibody binds to human NgR2 with a Kd of 2.3 nM and to rat NgR2 with a Kd of 0.48 nM (bivalent binding). The antibody binds specifically to residues 351-399 of NgR2, a member of the Nogo-66 receptor family which inhibits axonal growth and sporulating in the CNS.
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交叉反应
- 人, 大鼠
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产品特性
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Original Species of Ab: Rabbit
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- (His)6-tagged fragment of rat NgR2 (amino acids 279-420).
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亚型
- IgG kappa
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anti-Reticulon 4 Receptor-Like 2 (RTN4RL2) (AA 241-337) antibody
RTN4RL2 适用: 人, Cow ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 4 Receptor-Like 2 (RTN4RL2) (AA 355-372) antibodyRTN4RL2 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Reticulon 4 Receptor-Like 2 (RTN4RL2) (C-Term) antibodyRTN4RL2 适用: 人, 大鼠, 小鼠, 马, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- The original antibody was used for for an ELISA on the NgR2 antigen to confirm the binding of the antibody. This antibody was used for a western blot on lysates of 293F cells transfected with vectors for mammalian cell expression of human NgR1, NgR2, and NgR3. This antibody was used for flow cytometry on 293F cells transfected with human NgR1, NgR2, and NgR3 mammalian cell expression vectors. This antibody was used for surface plasmon resonance on NgR1 and NgR2 to determine its binding affinity for its antigen. The antibody was used for immunofluorescence on PC12 cells and PC12-NgR1 or PC12-NgR2. This antibody was used for immunoprecipitation on rat PC12 cell lines stably expressing full-length rat NgR1 or NgR2 (Hofer et al., 2008, PMID:17140598).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- RTN4RL2 (Reticulon 4 Receptor-Like 2 (RTN4RL2))
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别名
- NgR2
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背景
- Nogo-66 Receptor
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UniProt
- Q86UN3
抗原
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